Inflammatory Bowel Disease

Functional Plasmon-Activated Water Increases Akkermansia muciniphila Abundance in Gut Microbiota to Ameliorate Inflammatory Bowel Disease

/ CY Liu / 發表迴響

Chun-Chao Chang 1 2 3, Chih-Yi Liu 4 5, I-Chia Su 1, Yuarn-Jang Lee 1 2, Hsing-Jung Yeh 1 2, Wen-Chao Chen 1, Chih-Jui Yu 1, Wei-Yu Kao 1 2, Yu-Chuan Liu 6 7, Chi-Jung Huang 8 9

Affiliations
  1. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei 110, Taiwan.
  2. Division of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
  3. TMU Research Center for Digestive Medicine, Taipei Medical University, Taipei 110, Taiwan.
  4. Department of Pathology, Sijhih Cathay General Hospital, New Taipei 221, Taiwan.
  5. School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei 242, Taiwan.
  6. Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.
  7. Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, Taipei 110, Taiwan.
  8. Department of Medical Research, Cathay General Hospital, Taipei 106, Taiwan.
  9. Department of Biochemistry, National Defense Medical Center, Taipei 114, Taiwan.

ABSTRACT

Inflammatory bowel disease (IBD) is associated with dysbiosis and intestinal barrier dysfunction, as indicated by epithelial hyperpermeability and high levels of mucosal-associated bacteria. Changes in gut microbiota may be correlated with IBD pathogenesis. Additionally, microbe-based treatments could mitigate clinical IBD symptoms. Plasmon-activated water (PAW) is known to have an anti-inflammatory potential. In this work, we studied the association between the anti-inflammatory ability of PAW and intestinal microbes, thereby improving IBD treatment. We examined the PAW-induced changes in the colonic immune activity and microbiota of mice by immunohistochemistry and next generation sequencing, determined whether drinking PAW can mitigate IBD induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and dysbiosis through mice animal models. The effects of specific probiotic species on mice with TNBS-induced IBD were also investigated. Experimental results indicated that PAW could change the local inflammation in the intestinal microenvironment. Moreover, the abundance of Akkermansia spp. was degraded in the TNBS-treated mice but elevated in the PAW-drinking mice. Daily rectal injection of Akkermansia muciniphila, a potential probiotic species in Akkermansia spp., also improved the health of the mice. Correspondingly, both PAW consumption and increasing the intestinal abundance of Akkermansia muciniphila can mitigate IBD in mice. These findings indicate that increasing the abundance of Akkermansia muciniphila in the gut through PAW consumption or other methods may mitigate IBD in mice with clinically significant IBD.

Keywords: Akkermansia muciniphila; gut microbiota; inflammatory bowel disease; microbial biomarker; plasmon-activated water.

>Int J Mol Sci. 2022 Sep 28;23(19):11422. doi: 10.3390/ijms231911422.

14. Functional Plasmon-Activated Water Increases Akkermansia muciniphila Abundance in Gut Microbiota (參考著作)下載

The Periodontopathic Pathogen, Porphyromonas gingivalis, Involves a Gut Inflammatory Response and Exacerbates Inflammatory Bowel Disease

/ CY Liu / 發表迴響

Yu-Chen Lee 1, Chih-Yi Liu 2 3, Chia-Long Lee 4, Ruo-Han Zhang 5 6, Chi-Jung Huang 7 8, Ting-Lin Yen 6 7 9

Affiliations
  1. Department of Dentistry, Cathay General Hospital, Taipei 106, Taiwan.
  2. Division of Pathology, Sijhih Cathay General Hospital, New Taipei City 221, Taiwan.
  3. School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 242, Taiwan.
  4. Department of Internal Medicine, Cathay General Hospital, Taipei 106, Taiwan.
  5. Graduate School of Health Industry Management, Ching Kuo Institute of Management and Health, Keelung City 20301, Taiwan.
  6. Department of Oral Hygiene Care, Ching Kuo Institute of Management and Health, Keelung City 20301, Taiwan.
  7. Department of Medical Research, Cathay General Hospital, Taipei 106, Taiwan.
  8. Department of Biochemistry, National Defense Medical Center, Taipei 114, Taiwan.
  9. Department of Pharmacology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan.

ABSTRACT

Periodontal disease (PD) is one of the most prevalent disorders globally and is strongly associated with many other diseases. Inflammatory bowel disease (IBD), an inflammatory condition of the colon and the small intestine, is reported to be associated with PD through undetermined mechanisms. We analyzed taxonomic assignment files from the Crohn’s Disease Viral and Microbial Metagenome Project (PRJEB3206). The abundance of Porphyromonadaceae in fecal samples was significantly different between patients with Crohn’s disease and control volunteers. Dextran sulfate sodium was used to induce colitis in mice to reveal the effect of this periodontopathic pathogen in vivo. After intrarectal implantation of Porphyromonas gingivalis (Pg)-the primary pathogen causing PD-the disease activity index score, colonic epithelial loss, and inflammatory cell infiltration were intensified. In addition, tumor necrosis factor-α and interleukin-6 showed the highest levels in Pg-infected colons. This revealed the importance of Pg in the exacerbation of IBD. Thus, simultaneous treatment of PD should be considered for people with IBD. Moreover, implantation of Pg in the rectum worsened the clinical symptoms of colitis in mice. Because Pg participates in the pathogenesis of IBD, reducing the chances of it entering the intestine might prevent the worsening of this disorder.

Keywords: Porphyromonas gingivalis; gut inflammation; inflammatory bowel disease; periodontal disease.

>Pathogens. 2022 Jan 11;11(1):84. doi: 10.3390/pathogens11010084.

8. Periodontopathic Pathogen, Porphyromonas gingivalis, Involves a Gut Inflammatory Response (參考著作)下載